electroPioreactor v0.02.md

Bill of Materials

Pioreactor

1. 1x Pioreactor 20ml v1.1 with Raspberry Pi and Micro SD card, normally configured
2. 1x Dovetail platform for Raspberry Pi

Electrolysis

1. Either
   • 1x Platinum plated titanium anode (for MEP0.02Pt – 6mm diameter, 60mm total length, 35mm platinised length), or
   • 1x Graphite anode (for MEP0.02C – 6mm diameter, 60mm total length)
2. 1x Crocodile clip
3. 1x Stainless Steel 60mm M4 bolt
4. 1x Stainless Steel M4 Locking Nut
5. 1x Ring Crimp Terminal
6. 1x Pioreactor vial cap with hole for 6mm electrode
7. 4x Female Dupont Cables: Amazon.co.uk (also needed for CO₂ sparging)

CO₂ sparging

1. 1x Sodastream cylinder: Amazon.co.uk (classic blue NOT quick connect pink)
2. 1x Sodastream holder for Pioreactor
3. 1x W21.8-14 to TR21-4 adaptor: Amazon.co.uk ¹
4. 1x CO₂ Regulator and Solenoid: Amazon.co.uk ²
5. ~ 20cm 4mm PU tubing: Amazon.co.uk
6. 2x Check valves: Amazon.co.uk
7. 1x 3/16" barb male Luer lock: Amazon.co.uk
8. 1/16" barb Luer Locks - 2x Male: Amazon.co.uk & 2x Female: Amazon.co.uk
9. ~ 20cm 1/16" ID Silicone tubing: Amazon.co.uk
10. 1x 2.1mm x 5.5mm Male Barrel Plug: Amazon.co.uk - to connect Raspberry Pi to solenoid with:

Continuous Flow

1. 2x 12V peristaltic pump
2. 2x Dovetail peristaltic pump holder
3. 2x Centrifuge tubes: Amazon.co.uk
4. 2x Dovetail centrifuge tube holders

Reagents

• See this post: https://forum.amybo.org/d/22-hob-culture-medium-ingredients/7

Optional extras

1. Spare 20ml glass vials: Amazon.co.uk

Recommended Budget Labware

1. 1x 1-5ml Pipettor & tips
2. 1x Nitrile Gloves: Amazon.co.uk
3. 1x Spatula: Amazon.co.uk
4. 1x Weighing Boat: Amazon.co.uk
5. Jewellers Scales
6. 1x 1L volumetric flask: Labpals
7. 1x 1L Reagent Bottle: Labpals
8. 1x 1L Amber Reagent Bottle: Labpals
9. 1x 1L Wash Bottle: Labpals

Recommended Tools for Assembly

1. 1x Crimping Pliers: Amazon.co.uk
2. 1x 150mm Pliers Wrench: Amazon.co.uk
3. 1x Needle-Nose Pliers: Amazon.co.uk
4. 1x PH0 Screwdriver: Amazon.co.uk